Cloning Strategy

CD79A Linker Optimization

Antibody Choices 

Tuesday's To Do's 

• Sodium Azide solution
• Midi's:
  • hEF1a:CymR
  • hEF1a-CuO:mKate
  • CMV5-CuO:mKate
  • TRE:Syk
• Miniprep:
  • Golden gate donor mini product
  • LRs:
    • EGSH:mKate
    • hEf1a:VgEcR-2A-RXR
    • TRE:mKate
    • hEF1a:Gal4VP16 (remember which pDEST)
    • hEF1a:CD79B-TCS-Gal4VP16 
    • hEF1a:tagBFP
• digest:
  • hEF1a:CymR
  • hEF1a-CuO:mKate
  • CMV5-CuO:mKate 
  • TRE:Syk 
  • EGSH:mKate
  • hEf1a:VgEcR-2A-RXR
  • TRE:mKate
  • hEF1a:Gal4VP16 (remember which pDEST)
  • hEF1a:CD79B-TCS-Gal4VP16 
  • hEF1a:tagBFP
    
• Golden Gates
  
  • TRE:Syk-15-tTEVp
  • CD79A golden gates

Monday's To Do's 

• Oligomerize beta-amyloid
• Non-Lab To Do's:

• Order chicken lysozyme and gp120?
• order antibodies for antigen binding detection

Geneious To Do List:

• Antibody cloning for chicken lysozyme - Kathryn 
• hef1a sequencing primersBCR 2.0 To Do

^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

Higher Level To Do's:

• Decide on other antigen-variable regions we want to test  
• Is gantenerumab proprietary and can we actually publish with it?