Cloning Strategy

CD79A Linker Optimization

Antibody Choices 

STATUS TABLE

Monday's to do's

• Retransform B15T9
• B9T9 minipreps of cultures that are already in the fridge
• Midiprep (from freezer)
  • S9TEV
  • B15T15
• Get aliquot of pENTR VP16Gal4 from Jeremy and start LR
• Digest all of the Syk-TEVp and Syk-tTEVp midis just to be sure (since there's an internal cut site that didn't get read in sequencing)

Sunday's to do's

• Split cells

Saturday's to do's

• Replate B15T9

Friday's to do's

• Take B15T15 midiprep out of incubator
• Take B9T9 colonies out of incubator

Thursday's to do's

• Replate B15T9
• Take midipreps out of incubator
• Reinoculate B15T15 midi
• Inoculate more B9T9 colonies

Wednesday's to do's

• Split cells
• Golden gate/transform B15T9
• Midiprep
  • A15T9
  • B3T15
  • B9T15
  • B12T9
• Inoculate midiprep culture
  • S9TEV
  • B15T15

Sequencing account information:
^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

## Digest tTEVp and TEVp midis just to be sure (since there's an internal cut site)

Non-Lab To Do's 

• Bug Brian about ordering antibody testing supplies

Higher Level To Do's:

• Decide on other antigen-variable regions we want to test  
• Is gantenerumab proprietary and can we actually publish with it?