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BCR 2.0 

Experimental Progress:

Cloning Progress
Parts Tree
Cloning Strategy
Experiments
Linker Optimization
Antibody Choices 

 

Useful Links: 

Protocols
Immediate To Do's:
TaskClaimed byDate CompletedOther Notes
CD79A/B, Gal4VP16, RFP PCR try3CRApr 10

 

Gel verification of try3 PCR productLAApr 10RFP and Gal4 worked, CD79s didn't - we probably need to redesign primers
Redesign primersLA + CRApr 11CD79A F and R and CD79B F primers were redesigned and ordered
AarI golden gate of CD79A constructLAApr 30 
Take out CD79A golden gate platesLAMay 2 

Make LB

CRMay 4 

CD79A golden gate liquid cultures

LAMay 4

Cultures in at 6pm - need to be taken out by 10/11 am May 5

in 215 shaking incubator

Take out CD79A golden gate liquid culturesLAMay 5In 4C

CD79A golden gate minipreps

LAMay 6In yellow rack in 4C - need to be nanodropped

CD79A golden gate restriction verification

CRMay 6Some promising constructs
Send promising CD79A golden gates for sequencing   
  • CD79A linker golden gates
   
Redesign CD79B reverse PCR primerCRMay 24 
TaskClaimed byDate CompletedOther Notes
Order primers for PCRing constant region  primers on geneious
    

 

  • task dependent on above task
Higher Level To Do's:
  • Finish Discussing Figures for Experiment list
  • Decide on other antigen-variable regions we want to test 
  • Syk-Lyn experiment design 
  • Receptor dilution experiment design
  • Other experimental planning

 

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