Experimental Progress:

Cloning Progress

Parts Tree

Cloning Strategy

Experiments

Linker Optimization

Antibody Choices 

Useful Links: 

Protocols

Immediate To Do's:

• kill tret:vp16gal4 LR
• transform tret:vp16gal4 LR
• pick colonies from CD79A linker GG plates
• retransform 9TCS6
• pick colonies from LR transforms (pLac:mKate, h:LacI, tret:mKate
• CD79B-RFP-G4 SDM
• look over syk linker in silico cloning 
• update timeline

BCR 2.0 To Do

^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

Higher Level To Do's:

• Decide on other antigen-variable regions we want to test  
• Other experimental planning (on experimental page)
• How do we do site directed mutagenesis?
• Literature research: 
  • kozak sequences for heavy and light chains and CD79s
  • truncated TEVp
  • other antigens to detect using BCR (we were thinking of small molecule detection)
  • inducible promoters (we were considering LacI - an inducible repressor - but we want to look into other options first) 
• Cloning planning 
  • hEF1a:Syk-pTet:mRFP-TEVp
  • variable region