Old Todo lists
These will be deleted on 2015-06-29 (monday) unless I hear otherwise
Don't delete things - everything is up to date
Immediate To Do's:
- kill TRE:Gal4VP16 LR
- digests and gels for CD79A linker constructs
- run SDM on gel -> gel extraction
- send CD79A-XTCSX-G4 for sequencing
- transform TRE:Gal4VP16 LR
- transform CD79B-RFP-Gal4 SDM product
Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Other experimental planning (on experimental page)
- Parts list (excel sheet detailing specs/locations of parts)
- How do we do site directed mutagenesis?
- Literature research:
- kozak sequences for heavy and light chains and CD79s
- truncated TEVp
- other antigens to detect using BCR (we were thinking of small molecule detection)
- inducible promoters (we were considering LacI - an inducible repressor - but we want to look into other options first)
- Cloning planning
- hEF1a:Syk-pTet:mRFP-TEVp
- variable region