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Monday's To Do's 
  • pick more CD79A linker colonies (all old DNA is not good)
  • make more DNA of failed constructs from 07/03 gel
  • digest, gel pENTR mKate
  • LR EGSH:mKate, hEf1a:VgEcR-2A-RXR
  • sequence TRE:syk-ptet:mrfp-(t)TEVP
  • transform MAV1212 

 

CMV5-CuO 715:

hEF1a-CuO 715:09

CymR-CuO 710:09


Geneious To Do List:
  • Antibody cloning for chicken lysozyme - Kathryn 
  • hef1a sequencing primers

 

BCR 2.0 To Do

^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

 

 

Higher Level To Do's:
  • Decide on other antigen-variable regions we want to test  
  • Other experimental planning (on experimental page)
  • Parts list (excel sheet detailing specs/locations of parts)
  • How do we do site directed mutagenesis?
  • Literature research: 
    • kozak sequences for heavy and light chains and CD79s
    • truncated TEVp
    • other antigens to detect using BCR (we were thinking of small molecule detection)
    • inducible promoters (we were considering LacI - an inducible repressor - but we want to look into other options first) 
  • Cloning planning 
    • hEF1a:Syk-pTet:mRFP-TEVp
    • variable region 

 

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