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Friday's To Do's 
Thursday's To Do's
  • gel verify:
  • Pick colonies for midi
  • PCR purify TRE:Syk-15-tTEVp
  • Golden gate TRE:Syk-15-tTEVp
  • LR - probably can't do today because no LR clonase
    • EGSH:mKate
    • hEf1a:VgEcR-2A-RXR
    • TRE:mKate
    • hEF1a:Gal4VP16 (remember which pDEST)
    • hEF1a:CD79B-TCS-Gal4VP16 
Wednesday's To Do's
  • Flow cytometry with Brian (big grin)
  • Order chicken lysozyme and gp120?
  • Non-lab to do's
      • Design (or find?) sequencing primers for mIgM CH (constant region)
      • Plan experiment to test gantenerumab binding to beta-amyloid
      • Leader sequences for ordering gBlocks of variable regions?

CMV5-CuO 715:

hEF1a-CuO 715:09

CymR-CuO 710:09


Geneious To Do List:
  • Antibody cloning for chicken lysozyme - Kathryn 
  • hef1a sequencing primers

 

BCR 2.0 To Do

^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

 

 

Higher Level To Do's:
  • Decide on other antigen-variable regions we want to test  
  • Other experimental planning (on experimental page)
  • Parts list (excel sheet detailing specs/locations of parts)
  • How do we do site directed mutagenesis?
  • Literature research: 
    • kozak sequences for heavy and light chains and CD79s
    • truncated TEVp
    • other antigens to detect using BCR (we were thinking of small molecule detection)
    • inducible promoters (we were considering LacI - an inducible repressor - but we want to look into other options first) 
  • Cloning planning 
    • hEF1a:Syk-pTet:mRFP-TEVp
    • variable region 
  • Is gantenerumab proprietary and can we actually publish with it?

 

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