Friday's To Do's
- CD79B-RFP-G4 SDM
- Midiprep and digest
- hEF1a:tagBFP
- Inoculate minis
- RFP-IgM CH golden gate
- hEF1a:CD79A-linker-G4
- Miniprep and digest
- hEF1a:CD79A-linker-G4
- TRE:Syk-15-tTEV
- hEF1a:VP16Gal4
- EGSH:mKate
- hEF1a:VgEcR-2A-RxR
- hEF1a:CD79B-TCS-G4
- TRE:mKate
- Inoculate midis
- hEF1a:VP16Gal4
- EGSH:mKate
- hEF1a:VgEcR-2A-RxR
- hEF1a:CD79B-TCS-G4
- TRE:mKate
- Sequencing
- hEF1a:CD79A-linker-G4
- TRE:Syk-15-tTEV
- TRE:Syk-RFP-TEV (missing read only)
- TRE:Syk-RFP-tTEV (missing read only)
Thursday's To Do's
- Innoculate midi cultures
- hEF1a:tagBFP (from liquid mini culture)
- Inoculate mini cultures - because picking from plates of transformed reactions
- hEF1a:CD79A-linker-G4 golden gates
- TRE:Syk-15-tTEV
- TRE:mKate
- hEF1a:gal4VP16
- EGSH:mKate
- hEF1a:VgEcR-2A-RxR
- hEF1a:CD79B-TCS-Gal4vp16
- Transform
- RFP-IgM CH golden gate
- Failed CD79A linker golden gates
- Replate failed CD79A linker golden gates
- Re-golden gate failed golden gates?
Wednesday's To Do's
- Transform
- CD79A linker golden gates
- TRE:Syk-15-tTEVp golden gate
- EGSH:mKate
- hEF1a:VgEcR-2A-RxR
- hEF1a:gal4vp16
- TRE:mKate
- hEF1a:CD79B-TCS-Gal4vp16
- Design additional Syk-TEV Sequencing Primer
- order primers to re-pcr CD79B-RFP-G4
- Antibody PCRs
- Purify
- Gel
- Golden Gate
- Non-Lab To Do's:
- Order chicken lysozyme and gp120?
- order antibodies for antigen binding detection
Geneious To Do List:
- Antibody cloning for chicken lysozyme - Kathryn
hef1a sequencing primersBCR 2.0 To Do
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Is gantenerumab proprietary and can we actually publish with it?