Monday's To Do's
- Oligomerize AB
- Miniprep, digest, gel
- CD79A linker constructs
- Midis
- transform IgM-RFP golden gate
- golden gate TRE:Syk-15-TEVp in gg donr
Saturday's To Do's
- Midipreps
- CD79A golden gate T4 ligation
- Transformations
- CD79A golden gates
- IgM RFP golden gate
- Sequencing
- 3T3
- 6T6
- 6T12
- 9T3
Friday's To Do's
- Inoculate minis
- RFP-IgM CH golden gate
- hEF1a:CD79A-linker-G4
- Miniprep and digest
- hEF1a:CD79A-linker-G4
- TRE:Syk-15-tTEV
- hEF1a:VP16Gal4
- EGSH:mKate
- hEF1a:VgEcR-2A-RxR
- hEF1a:CD79B-TCS-G4
- TRE:mKate
- Inoculate midis
- hEF1a:VP16Gal4
- EGSH:mKate
- hEF1a:VgEcR-2A-RxR
- hEF1a:CD79B-TCS-G4
- TRE:mKate
- hEF1a:tagBFP
- Sequencing
- hEF1a:CD79A-linker-G4
- TRE:Syk-15-tTEV
- TRE:Syk-RFP-TEV (missing read only)
- TRE:Syk-RFP-tTEV (missing read only)
Thursday's To Do's
- Innoculate midi cultures
- hEF1a:tagBFP (from liquid mini culture)
- Inoculate mini cultures - because picking from plates of transformed reactions
- hEF1a:CD79A-linker-G4 golden gates
- Pick some for growth at 37C and others for growth at 30C
- TRE:Syk-15-tTEV - pick a couple of these (it's a golden gate with no screening)
- TRE:mKate
- hEF1a:gal4VP16
- EGSH:mKate
- hEF1a:VgEcR-2A-RxR
- hEF1a:CD79B-TCS-Gal4vp16
- Digest, gel:
- TRE:Syk-15-tTEV - pick a couple of these (it's a golden gate with no screening)
- TRE:mKate
- hEF1a:gal4VP16
- EGSH:mKate
- hEF1a:VgEcR-2A-RxR
- hEF1a:CD79B-TCS-Gal4vp16
- Transform
- RFP-IgM CH golden gate
- Replate failed CD79A linker golden gates
- Ordering things
- Chicken lysozyme, gp120
- Antibodies for antigen binding detection
- Cumate?
Geneious To Do List:
- Antibody cloning for chicken lysozyme - Kathryn
hef1a sequencing primersBCR 2.0 To Do
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
Non-Lab To Do's
- Cytometry time
- Order Cumate
- Chicken lysozyme, gp120
- Antibodies for antigen binding detection
Waiting on primers to do list:
- TRE:Syk-15-TEVp
- CD79B-mRFP-G4 PCR
Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Is gantenerumab proprietary and can we actually publish with it?