Tuesday's To Do's
- Gel for oligo annealing
- Mix new annealing buffer
- Anneal oligos
- Design IgM Sequencing Primers
- CD79B, gal4 PCR
- Golden Gates:
- hEF1a:CD79B-pTET:mRFP-Gal4 golden gate
- TRE:Syk-15-tTEVp
- Transform
- hEF1a:CD79B-pTET:mRFP-Gal4 GG
- TRE:Syk-15-tTEVp GG
- TRE:Syk-pTET:mRFP-tTEVp sequencing
- Pick pENTR RFP-IgM Colony
Sequencing account information:
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
Non-Lab To Do's
- Cytometry time
- Order Cumate
- Chicken lysozyme, gp120
- Antibodies for antigen binding detection
Waiting on primers to do list:
- TRE:Syk-15-TEVp
- CD79B-mRFP-G4 PCR
Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Is gantenerumab proprietary and can we actually publish with it?