Thursday's To Dos
- Transform golden gates - pick colonies later in the day
- Minipreps - linkers
- Midipreps - ggdonr and a-3t3
- Inoculate midipreps?
- Split cells
Wednesday's to Dos
- Redigest basically everything from yesterday and gel
- Pick colonies
- TRE:Syk-15-tTEV
- hEF1a:CD79B-RFP-G4
- pENTR VRC01 heavy
- pENTR VRC01 light
- pENTR HyHEL-10 heavy
- pENTR HyHEL-10 light
- Fix - see here for ideas
- TRE:Syk-15-tTEV
- hEF1a:CD79B-RFP-G4
- Syk linkers
- Miniprep andn digest
- CD79A-3T15
- CD79A-12T9
- CD79A-15T9
- Inoculate midipreps if the sequencing comes back good
- Regolden gate things - extra ligation for BsaI golden gate
Sequencing account information:
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
Non-Lab To Do's
- Cytometry time
- Order Cumate
- Chicken lysozyme, gp120
- Antibodies for antigen binding detection
Waiting on primers to do list:
Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Is gantenerumab proprietary and can we actually publish with it?
THINGS TO NOT FORGET TO DO:
- SYK-15tTEVP golden gate when we figure out what's wrong with the AarI
- 3T3 MIDI