BCR 2.0
Switchable Antigen-Sensing System (S.A.S.S.)
Sunday's To Do's
- Take out plates
- Miniprep (if you can find 2mL tubes...)
- pENTR HyHEL10 Heavy
- hEF1a:CD79B-9T15-G4
- Pick colonies
- Plated 8/8
- A-15T9
- TRE:Syk-3-TEVp
- TRE:Syk-6-TEVp
- TRE:Syk-9-TEVp
- TRE:Syk-12-TEVp
- B-3T15
- B-9T9
- TRE:Syk-15-tTEVp
- Streaked or restreaked 8/8
- A-3T15
- B-15T15
- B-15T9
- B-6T12
- Plated previously
- A-9T15
- Plated 8/8
- Start midiprep cultures
- B3T3
- B3T9
- B6T6
- B9T3
- B9T6
- B12T6
- B15T9
- Send for sequencing
- Minipreps (if they get done)
- pENTR HyHEL10 Heavy
- hEF1a:CD79B-9T15-G4
- CD79B linkers (#1 from before)
- B9T12
- B6T9
- Minipreps (if they get done)
Saturday's To Do's
- Kill LR (TRE:Syk-15-tTEVp)
- Take out plates + colonies
- Transform
- hEF1a:CD79A-15T9-G4 (because even though we midi'ed it, sequencing was bad)
- TRE:Syk-linker-TEVp (3, 6, 9, 12)
- hEF1a:CD79B-3T15-G4
- hEF1a:CD79B-9T9-G4
- TRE:Syk-15-tTEVp
- Streaked out
- A-3T15
- B-15T15
Sequencing account information:
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
Non-Lab To Do's
- Cytometry time
- Order Cumate
- Chicken lysozyme, gp120
- Antibodies for antigen binding detection
Waiting on primers to do list:
Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Is gantenerumab proprietary and can we actually publish with it?
THINGS TO NOT FORGET TO DO:
- SYK-15tTEVP golden gate when we figure out what's wrong with the AarI
- 3T3 MIDI