Cloning Strategy

CD79A Linker Optimization

Antibody Choices 

Thursday's to do's 

• Midis:
  • B9T12
  • B15T3
  • S9TEV
  • S12TEV
• Minis, sequencing 
  • B6T9
  • A9T15
• Inoculate mini
  • B15T9
  • B15T15
• Inoculate midi:
• Sequencing:
  • B6T9-1
  • A9T15-2
  • A3T15-2
• Restreak:
  • B15T9

Sequencing account information:
^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

Check on status:

• B9T12 - send for seq
• B6T9 - send for seq
• B12T9 - repeat seq
• B12T12 - repeat seq

Non-Lab To Do's 

• Cytometry time 
• Order Cumate 
• Chicken lysozyme, gp120
• Antibodies for antigen binding detection

Waiting on primers to do list:

Higher Level To Do's:

• Decide on other antigen-variable regions we want to test  
• Is gantenerumab proprietary and can we actually publish with it?

THINGS TO NOT FORGET TO DO:

• SYK-15tTEVP golden gate when we figure out what's wrong with the AarI
• 3T3 MIDI