Monday's To Do's
- pick more CD79A linker colonies (all old DNA is not good)
- make more DNA of failed constructs from 07/03 gel
- digest, gel pENTR mKate
- LR EGSH:mKate, hEf1a:VgEcR-2A-RXR
- sequence TRE:syk-ptet:mrfp-(t)TEVP
- transform MAV1212
CMV5-CuO 715:
hEF1a-CuO 715:09
CymR-CuO 710:09
Geneious To Do List:
- Antibody cloning for chicken lysozyme - Kathryn
hef1a sequencing primers
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Other experimental planning (on experimental page)
- Parts list (excel sheet detailing specs/locations of parts)
- How do we do site directed mutagenesis?
- Literature research:
- kozak sequences for heavy and light chains and CD79s
- truncated TEVp
- other antigens to detect using BCR (we were thinking of small molecule detection)
- inducible promoters (we were considering LacI - an inducible repressor - but we want to look into other options first)
- Cloning planning
- hEF1a:Syk-pTet:mRFP-TEVp
- variable region