For priority: 1 = top priority (absolutely necessary), 2 = middle priority (would be useful), 3 = low priority (only if we have time)
Name conventions
pENTR L1-Gene name-L2
pEXPR Promoter:Gene
Status
| Word | Meaning |
|---|---|
| in planning | being designed in geneious |
| designed | plasmid design completed in geneious |
| in lab | someone else in the lab has it |
| ordered | |
| in construction | |
| constructed | |
| in storage | we have it in our fridge/freezer, etc |
| NAME | Dependency | GROUP | STATUS | TYPE | LOCATION | INITIALS | CONSTRUCTED ON | OBTAINED FROM | PRIORITY | NOTES |
|---|---|---|---|---|---|---|---|---|---|---|
| pEXPR hEF1a: Gmab Heavy | pENTR Gmab Heavy pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR hEF1a: Gmab Light | pENTR Gmab Light pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR hEF1a: CD79A | pENTR CD79A pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR hEF1a: CD79B | pENTR CD79B pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR hEF1a: CD79A-TCS-Gal4VP16 | pENTR CD79A-TCS-Gal4VP16 pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR hEF1a: CD79B-TCS-Gal4VP16 | pENTR CD79B-TCS-Gal4VP16 pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR hEF1a: Lyn | pENTR Lyn pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR PTet: Lyn-mKate | pENTR Lyn-mKate pENTR PTet | Antibodies | designed | expression | ||||||
| pEXPR PTet: Syk-eYFP | pENTR Syk-eYFP pENTR PTet | Antibodies | designed | expression | ||||||
| pEXPR PTet: Syk-TEVp | pENTR Syk-TEVp pENTR PTet | Antibodies | designed | expression | ||||||
| pEXPR PTet: eBFP | pENTR eBFP | Antibodies | In Freezer | expression | ||||||
| pEXPR Gal4/UAS: mKate | - | Antibodies | In Freezer | expression | iGEM -20 | CR | N/A | Velia | High | |
| pEXPR hEF1a: rtTA | pENTR rtTA | Antibodies | In Freezer | expression | iGEM -20 | CR | N/A | Velia | High | |
| pEXPR Gal4UAS:eYFP | - | Antibodies | in someone else's freezer | expression | ||||||
| pEXPR Tre-TEVp | - | Antibodies | in someone else's freezer | expression | ||||||
| pENTR Gmab Heavy | gBlock mIgM Gmab Heavy | Antibodies | Planned | entry | ||||||
| pENTR Gmab Light | gBlock mIgM Gmab Light | Antibodies | Planned | entry | ||||||
| pENTR CD79A (pDONR223) | - | Antibodies | in freezer | entry | ||||||
| pENTR CD79B (pDONR223) | - | Antibodies | in freezer | entry | ||||||
| pENTR CD79A-TCS-Gal4VP16 | pENTR CD79A pENTR Gal4VP16 | Antibodies | Planned | entry | ||||||
| pENTR CD79B-TCS-Gal4VP16 | pENTR CD79B pENTR Gal4VP16 | Antibodies | Planned | entry | ||||||
| pENTR Lyn (pDONR223) | - | Antibodies | In culture | entry | ||||||
| pENTR Lyn-mKate | pENTR Lyn pENTR mKate | Antibodies | Planned | entry | ||||||
| pENTR Syk (pDONR223) | - | Antibodies | in culture | entry | ||||||
| pENTR Syk-eYFP | pENTR Syk pENTR eYFP | Antibodies | Planned | entry | ||||||
| pENTR Syk-TEVp | pENTR Syk pEXPR Tre-TEVp | Antibodies | Planned | entry | ||||||
| pENTR eBFP | - | Antibodies | ? | entry | ||||||
| pENTR mKate | - | Antibodies | ? | entry | iGEM -20 | CR | N/A | Velia | High | |
| pENTR rtTA | - | Antibodies | ? | entry | iGEM -20 | CR | N/A | Velia | High | |
| pENTR eYFP | - | Antibodies | ? | entry | ||||||
| pENTR Gal4VP16 | - | Antibodies | ? | entry | ||||||
| gBlock mIgM Gmab Heavy | - | Antibodies | ordered | gBlock | Ordered from IDT | |||||
| gBlock mIgM Gmab Light | - | Antibodies | ordered | gBlock | ||||||
| pENTR Tre-TEVp | Treatment | in the lab already | ||||||||
| Treatment | planned | ||||||||
| Treatment | to be ordered | ||||||||
| Treatment | in the lab already | ||||||||
| Treatment | in the lab already | ||||||||
| pEXPR U6:mKate-miRNA(intronic) | Treatment | planned | ||||||||
| pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic) | Treatment | planned | ||||||||
| Experiment 1 | ||||||||||
| Receptor | in storage | plasmid | +4 in iGEM room | LA | - | Kyle | not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enough | ||
| Receptor | in storage | plasmid | +4 in iGEM room | LA | - | Brandon | will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock | ||
| Receptor | in lab | plasmid | will be used to make LilrB2 expression vector | ||||||
| Receptor | in lab | plasmid | will be used to make LilrB2 expression vector | ||||||
| Receptor | in construction | plasmid | waiting on entry vector | ||||||
| Receptor | in construction | plasmid | ifailing to get liquid cultures to grow in order to miniprep to extract plasmid DNA | ||||||
| Receptor | in storage | gBlock | -20 in iGEM room | LA | - | IDT | used in Golden Gate | ||
| Receptor | in construction | waiting on entry vector | |||||||
| Receptor | in construction | failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA | |||||||
| Receptor | in storage | gBlock | -20 in iGEM room | LA | - | IDT | used in Golden Gate | ||
| Receptor | in storage | gBlock | -20 in iGEM room | LA | - | IDT | used in Golden Gate | ||
| PDONR MAV1212 | - | miRNA sensing | in storage | donor | -20 in iGEM room | RR | N/A | Jeremy Gam | High | Original Multi Assembly Vector, currently under cloning |
| pENTR L1_hEF1A_L2 | - | miRNA sensing | in lab? | entry | ? | RR | N/A | N/A | High | Stock in iGEM room may exist, but the one I found was empty |
| pENTR R1_l7ae_L4 | - | miRNA sensing | in storage | entry | -20 in iGEM room | RR | N/A | N/A | High | |
| pEXPR hEF1A:l7ae | MAV1212 | miRNA sensing | designed | expression | N/A | RR | N/A | N/A | High | |
| pENTR R1_mKate_L4 | - | miRNA sensing | in storage | entry | -20 in iGEM room | RR | N/A | N/A | High | |