Title: Protocols  
Author: Unknown User (jamesa@mit.edu) Mar 07, 2014 17:47
Last Changed by: Unknown User (lylaatta@mit.edu) Nov 04, 2015 14:08
Tiny Link: (useful for email) https://wikis.mit.edu/confluence/x/PI6ABQ
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MIT iGEM 2014 (3)
    Page: Inducible upregulation of BACE2 and YFP
    Page: BCR2.0
    Home page: Home
Hierarchy
Parent Page
    Page: Useful References
Children (45)
    Page: Annealing and Kinasing Oligos
    Page: Attatching B-Cells using Poly-L-Lysine
    Page: Autoclave
    Page: Best Practices for Tissue Culture Success!
    Page: Beta Amyloid Streptavidin Staining Protocol for Cytometry
    Page: Beta Amyloid Streptavidin Staining Protocol for Live Microscopy
    Page: BP Reaction
    Page: Cell Culture Media
    Page: Cookie preparation
    Page: Co-transfection of HEK293 with Lipofectamine 2000 (24 well plate)
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Nov 04, 2015 14:08 Unknown User (lylaatta@mit.edu) View Changes
Jul 06, 2015 15:07 Unknown User (lylaatta@mit.edu) View Changes
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May 27, 2015 11:20 Unknown User (clrichar@mit.edu)  
Outgoing Links
External Links (2)
    www.abcam.com/index.html?pageconfig=resource&rid=11449
    www.abcam.com/protocols/indirect-flow-cytometry-protocol
MIT iGEM 2014 (40)     Page: Best Practices for Tissue Culture Success!
    Page: Immunostaining for Fluorescent Microscopy
    Page: Autoclave
    Page: Western Blot
    Page: Primer Design
    Page: Mutating DNA Sequences to Get Rid of Cut Sites
    Page: Midiprep
    Page: Fusion proteins and Geneious
    Page: Beta Amyloid Streptavidin Staining Protocol for Cytometry
    Page: Co-transfection of HEK293 with Lipofectamine 2000 (24 well plate)
    Page: Designing and Ordering Primers
    Page: Make Cell Stock
    Page: Cell Culture Media
    Page: Making S.O.C.
    Page: Cookie preparation
    Page: Restriction Digest
    Page: Transfection (Lipofectamine 2000)
    Page: Oligomerizing Beta Amyloid
    Page: Splitting Cells & Seeding Plates
    Page: BP Reaction
    Page: Pouring GGDonr Plates
    Page: PCR Purification
    Page: Protocols
    Page: Attatching B-Cells using Poly-L-Lysine
    Page: Gel
    Page: Annealing and Kinasing Oligos
    Page: Miniprep
    Page: Transfection (using Lipofectamine LTX)
    Page: LR (Gateway)
    Page: Beta Amyloid Streptavidin Staining Protocol for Live Microscopy
    Page: FACS Cell Prep (24-well plate)
    Page: PCR
    Page: Freezing and Thawing HEK293 Cells
    Page: Transformation
    Page: Golden Gate
    Page: Locating a Q cut site within a gene
    Page: Resuspending PCR Primers
    Page: Immunostaining for Flow Cytometry
    Page: Preparing LB Agar Plates
    Page: Making Liquid Culture