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DateSummary
Anchor
6
6
06/01
  •  h:Lyn-TEV Colonies Picked
  • h:CDB-RFP-G4 transformation
  • h:CDA-RFP-G4 Sequence Analysis
  • Inocculate Midipreps
  • h:Lyn, h:CDB-TCS-G4 cryostocks
06/02
  • Cloning Redesign
  • Pick hEF1a:CD79B-pTet:RFP-Gal4VP16 Colonies
06/03
  • Miniprep hEF1a:CD79B-pTet:RFP-Gal4VP16
  • Cryostock hEF1a:CD79A-pTet:RFP-Gal4VP16
06/04
  • Glycerol Stock hEF1a:CD79A-pTet:RFP-Gal4VP16
  • Midiprep 4 Frozen pellets
  • Digest & Gel hEF1a:CD79B-pTet:RFP-Gal4VP16
06/08
  • hEF1a:CD79B-pTet:RFP-Gal4VP16 Digest & gel
06/09
  • Send for sequencing
06/11
  • Sequencing Analysis
  • Glycerol Stock H:CDA-RFP-G4
06/16
  • designed SDM primers
  • LR LacI:BCR
  • experimental design + deadlines