-
Created by Unknown User (lylaatta@mit.edu), last modified by Unknown User (clrichar@mit.edu) on May 31, 2015 22:30
You are viewing an old version of this page. View the current version.
Compare with Current
View Page History
« Previous
Version 21
Next »
| Date | Summary |
|---|
| 03/23/2015 | Verification of linker-switchable PCRs |
| 04/01 | - rePCRs of CD79B, Gal4 and RFP to make linker switchable constructs
- Gel verification of 03/31 and 04/01 PCRs
|
| 04/10 | - rePCRs of CD79A/B, Gal4 and RFP to make linker switchable constructs
- Gel verification of PCRs
|
| 04/11 | redesign CD79A/B primers |
| 04/25 | - rePCRs of CD79A/B, to make linker switchable constructs
- Gel verification of PCRs
|
| 04/30 | - rePCR of CD79B with DMSO
- gel purification of CD79A
- PCR purification of pTet:mRFP and Gal4VP16
- AarI golden gate to make hEF1a:CD79A-pTet:mRFP-Gal4VP16
|
| 05/01 | - Transformation of hEF1a:CD79A-pTET:mRFP-Gal4VP16 golden gate
|
| 05/04 | - hEF1a:CD79A-pTet:mRFP-Gal4VP16 liquid culture
|
| 05/06 | - hEF1a:CD79A-pTet:mRFP-Gal4VP16 miniprep
- hEF1a:CD79A-pTet:mRFP-Gal4VP16 restriction digest verification
|
| 05/29 | - Transformations for Glycerol Stocks
- Sent for hEF1a:CD79B-TCS-Gal4VP16 sequencing
- PCRd CD79B
- CD79B Gel
|
| 05/30 | - CD79B Gel Extraction (Failed)
- Midi Cultures Inocculated
- CD79B PCR 2
- Picked Colonies for H:Lyn, H:LT, H:CB-TCS-G4
|
| 05/31 | - CD79B PCR Gel
- CD79B Gel Extraction
- hEF1a:CD79B-pTet:RFP-Gal4VP16 Golden Gate
- Lyn-TEV colonies picked
|